The fold change values derived from the RNA-seq analysis of DEGs are compared with those obtained by qRT-PCR determined by 2−ΔΔCT. (F) Correlation between qRT-PCR and RNA-seq results for select DEGs. (E) Average heart rate of 60 hpf WT and slc38a9 mutant zebrafish larvae. The lower terms are more specific, while the upper terms are more general. (D) The directed acyclic graph (DAG) of biological process. (C) Gene ontology (GO) analysis of differentially expressed genes in biological processes (red column), cellular component (green column), and molecular function (blue column). The red dot shows upregulated genes, the green dot shows downregulated genes, and the blue dot shows an unchanged gene. (B) Volcano plot of differential expression analysis of slc38a9 mutant and control larvae showing the relationship between −log10(padj) and log2FoldChanges. Principal component 1 (PC1), principal component 2 (PC2), and principal component 3 (PC3) were used for analysis. (A) Principal component analysis (PCA) plot of four wild type and slc38a9 mutant RNA-seq datasets. RNA sequencing analysis of slc38a9 mutant zebrafish.
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